| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.182902 |
| Chromosome: | chromosome 6 |
| Location: | 916685 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255100 | (1 of 4) IPR000533 - Tropomyosin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGTGTCCACAAGGAAATAGGGTCACGC |
| Internal bar code: | CTCAAGCACGGGCAGCCTTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 4.34783 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGACAGTGGTAAGGCAG |
| Suggested primer 2: | CAGAGCTTAACGAACCCTGC |