| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.182910 |
| Chromosome: | chromosome 8 |
| Location: | 89820 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358528 | FAP157 | Flagellar Associated Protein 157 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAATGATCCCCCCCCCCCCCGCCATAG |
| Internal bar code: | AAACGAGCTACCTTATATTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 548 |
| LEAP-Seq percent confirming: | 98.0992 |
| LEAP-Seq n confirming: | 1187 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCAAAGTCCTTGGTC |
| Suggested primer 2: | GGTGGTGGCGCTCTATGTAT |