Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.182910 |
Chromosome: | chromosome_8 |
Location: | 89820 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g358528 | FAP157 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CATGAATGATCCCCCCCCCCCCCGCCATAG |
Internal bar code: | AAACGAGCTACCTTATATTTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 548 |
LEAP-Seq percent confirming: | 98.0992 |
LEAP-Seq n confirming: | 1187 |
LEAP-Seq n nonconfirming: | 23 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTGCTCAAAGTCCTTGGTC |
Suggested primer 2: | GGTGGTGGCGCTCTATGTAT |