| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182910 |
| Chromosome: | chromosome_8 |
| Location: | 89822 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g358528 | FAP157 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TAGCTAAGAAAATGATGGGGGGGCAGGCGC |
| Internal bar code: | AGGGGACGGTTGGTGGGGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 389 |
| LEAP-Seq percent confirming: | 97.3955 |
| LEAP-Seq n confirming: | 1608 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTCAAAGTCCTTGGTC |
| Suggested primer 2: | GGTGGTGGCGCTCTATGTAT |