| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.182934 |
| Chromosome: | chromosome 6 |
| Location: | 1062661 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256500 | (1 of 2) PTHR14187//PTHR14187:SF5 - ALPHA KINASE/ELONGATION FACTOR 2 KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCAGCACCTGCGTCGGGTGCATGCAT |
| Internal bar code: | AGCTGACCCTGGGAATGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 971 |
| LEAP-Seq percent confirming: | 96.8596 |
| LEAP-Seq n confirming: | 3331 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTAGCGCTCTGTGACGGTT |
| Suggested primer 2: | CACAATCAATGGTGCTGGTC |