| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.182948 |
| Chromosome: | chromosome_2 |
| Location: | 4856244 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g103250 | MRP5 | ABC transporter, multidrug resistance associated protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGTCCCAGGAGGCGAGAGTGATGTGTGCT |
| Internal bar code: | CGCGATCAGCGCGCCTGTAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 80.5068 |
| LEAP-Seq n confirming: | 413 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTCGCTTTCCGATATCAT |
| Suggested primer 2: | CATACATCGCATTGTTTCGG |