Insertion junction: LMJ.RY0402.182948_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.182948
Chromosome:chromosome 2
Location:4856244
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g103250 MRP5 (1 of 3) PTHR24223//PTHR24223:SF252 - FAMILY NOT NAMED // PROTEIN MRP-2; ABC transporter, multidrug resistance associated protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGTCCCAGGAGGCGAGAGTGATGTGTGCT
Internal bar code:CGCGATCAGCGCGCCTGTAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:223
LEAP-Seq percent confirming:80.5068
LEAP-Seq n confirming:413
LEAP-Seq n nonconfirming:100
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTCGCTTTCCGATATCAT
Suggested primer 2:CATACATCGCATTGTTTCGG