Insertion junction: LMJ.RY0402.182948_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.182948
Chromosome:chromosome 2
Location:4856427
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g103250 MRP5 (1 of 3) PTHR24223//PTHR24223:SF252 - FAMILY NOT NAMED // PROTEIN MRP-2; ABC transporter, multidrug resistance associated protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTACGGCAGCTGCCCAGTGCAACACGGCC
Internal bar code:CCAGAATTGGTCGACGATTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:260
LEAP-Seq percent confirming:99.7733
LEAP-Seq n confirming:2201
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTCGCTTTGCTTCACTC
Suggested primer 2:CTGGATGAGGAAAGTCCCAA