Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.182972 |
Chromosome: | chromosome_13 |
Location: | 1792803 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g574900 | FAP228,GTR2 | Glycosyltransferase | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TACAAGGGTATCTCGCAGAAAGTAAGGTTC |
Internal bar code: | TGAAAGTTGAAGGTATGATCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 332 |
LEAP-Seq percent confirming: | 99.0164 |
LEAP-Seq n confirming: | 302 |
LEAP-Seq n nonconfirming: | 3 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCGTGTCAGCAATCTCTCAA |
Suggested primer 2: | GATAGGGCTGCTGCTGGTAG |