| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183000 |
| Chromosome: | chromosome_3 |
| Location: | 5258324 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g183200 | CPC1 | Protein associated with central pair microtubule complex | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACGACAGGTAAGCCCCCAGCACTGGCGCAC |
| Internal bar code: | TTGGCACACTCCGATGAATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 94.9666 |
| LEAP-Seq n confirming: | 1849 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGGCCGACCAGATAGA |
| Suggested primer 2: | TCTTCTCAGTGACAGCCGTG |