Insertion junction: LMJ.RY0402.183004_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.183004
Chromosome:chromosome 6
Location:3763688
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g278157 (1 of 1) 3.2.1.143 - Poly(ADP-ribose) glycohydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCTACTTCGCCTCCCCCGGCTGGGCGGC
Internal bar code:TCTTGCTTCAGCATCAGGCTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:814
LEAP-Seq percent confirming:99.6905
LEAP-Seq n confirming:5476
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTTAAGCACTCGCAGGAA
Suggested primer 2:ACCTTATCATCCGTGGCTTG