| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183014 |
| Chromosome: | chromosome 9 |
| Location: | 3135342 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386949 | (1 of 17) IPR015813 - Pyruvate/Phosphoenolpyruvate kinase-like domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACGCAAGCGACGCGTGCAGCTGACCGG |
| Internal bar code: | CTCCCCTTGCCTCTGCCAGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 401 |
| LEAP-Seq percent confirming: | 99.5937 |
| LEAP-Seq n confirming: | 8579 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTATGCGACAGAAGGCAG |
| Suggested primer 2: | GCAAGCCTGCTGGTAAAAAG |