| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183021 |
| Chromosome: | chromosome 12 |
| Location: | 5881292 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGCGGACGACGCTGTGTGTTAGAAAGC |
| Internal bar code: | AATTATTGTTCACATATTGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1013 |
| LEAP-Seq percent confirming: | 99.4513 |
| LEAP-Seq n confirming: | 3625 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTGAGCTTGACGCATTTC |
| Suggested primer 2: | ATCACAAGGAAGGCAGTTGG |