| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.183034 |
| Chromosome: | chromosome 13 |
| Location: | 2628695 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581450 | RPN7 | (1 of 1) K03037 - 26S proteasome regulatory subunit N7 (PSMD6, RPN7); 26S proteasome regulatory subunit | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGGGCGCGGCCCGAACGCCACGTCGGA |
| Internal bar code: | GAACAAGGTTACGTTCGCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 850 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCGGTAACGCTTGATGTT |
| Suggested primer 2: | CTCCACAACGCTTGTCTCAA |