| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183036 |
| Chromosome: | chromosome 11 |
| Location: | 1633666 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467768 | (1 of 1) K10277 - lysine-specific demethylase 8 [EC:1.14.11.27] (KDM8, JMJD5) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGAGCGAGTGCGCTTCCAGTCACGTCCT |
| Internal bar code: | GCGCGACTAGGAATCAATCTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 99.571 |
| LEAP-Seq n confirming: | 6267 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTGAGGGTCATGAGCTG |
| Suggested primer 2: | GTTACTGCGGTAAAGGCACG |