| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183043 |
| Chromosome: | chromosome 16 |
| Location: | 3595617 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682725 | GST2 | Glutathione S-transferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCGCCTCACGCCCCCGCCTCACGCCCC |
| Internal bar code: | GAATTCCACTGATCGAGGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 78.4431 |
| LEAP-Seq n confirming: | 262 |
| LEAP-Seq n nonconfirming: | 72 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCTACTTCCGCTCCAAA |
| Suggested primer 2: | GCATAAGCGACACACCACAC |