| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183078 |
| Chromosome: | chromosome 12 |
| Location: | 3033879 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500715 | (1 of 5) 6.2.1.3 - Long-chain-fatty-acid--CoA ligase / Lignoceroyl-CoA synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAGCGGTGCAAATGTGTCTTCGTGCGCT |
| Internal bar code: | CGAATTTGTGATTCCATGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 224 |
| LEAP-Seq percent confirming: | 84.6966 |
| LEAP-Seq n confirming: | 321 |
| LEAP-Seq n nonconfirming: | 58 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCCTGTGCCTCTCTTCCT |
| Suggested primer 2: | GGAGGAGGAGGACGAGCTAC |