| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183079 |
| Chromosome: | chromosome 9 |
| Location: | 24836 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g386119 | (1 of 2) PF13383 - Methyltransferase domain (Methyltransf_22) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACTCAAAGCCTGCAAGCATGTAGATGA |
| Internal bar code: | GTCGGCCTAGGCCCTAGTTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 99.3956 |
| LEAP-Seq n confirming: | 2138 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCAACAGGTCCCTGCAAT |
| Suggested primer 2: | ATTAGTCGCCAGACCCACAC |