| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183084 |
| Chromosome: | chromosome 1 |
| Location: | 5948059 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g042352 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGGCGCCGGGCTGGGAGACACCGGCGA |
| Internal bar code: | AAAGCGGCAGTAATCGGGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 15 |
| LEAP-Seq percent confirming: | 56.4972 |
| LEAP-Seq n confirming: | 100 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGAGGAACAGGCGAC |
| Suggested primer 2: | GTCGCCTGTTCCTCCCAG |