Insertion junction: LMJ.RY0402.183112_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.183112
Chromosome:chromosome_12
Location:1838533
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g511850 GSK3 Glycogen Synthase Kinase antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):TTGACACGCGTTGAACATAACGCCCAAACC
Internal bar code:CACGGGCTCCTGTGCCAGGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1048
LEAP-Seq percent confirming:99.2802
LEAP-Seq n confirming:1793
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACAGATACCAGCGTGAA
Suggested primer 2:TCTGGGAGTAGCGCGTAGTT