| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183115 |
| Chromosome: | chromosome 7 |
| Location: | 2891027 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332450 | (1 of 1) PTHR31528//PTHR31528:SF1 - FAMILY NOT NAMED // PYRIMIDINE PRECURSOR BIOSYNTHESIS ENZYME THI11-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAACGGTCGGCGCAATCGGACCTTTCCG |
| Internal bar code: | TTCACTGTGATTAGTGCGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1014 |
| LEAP-Seq percent confirming: | 99.6691 |
| LEAP-Seq n confirming: | 6626 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACCAGTCACGATCACAA |
| Suggested primer 2: | CGGCCACCTACATAACGAGT |