| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183137 |
| Chromosome: | chromosome 2 |
| Location: | 1021768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080500 | (1 of 5) 2.4.1.17 - Glucuronosyltransferase / Uridine diphosphoglucuronyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATTCTAAGATTGCTGGCACGCGTACCCG |
| Internal bar code: | TGACTTCTCGCCAACCGACCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 822 |
| LEAP-Seq percent confirming: | 98.3051 |
| LEAP-Seq n confirming: | 1682 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAAGCTTAGGAAGGTGC |
| Suggested primer 2: | TGACGATGACGAAGACGAAG |