| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183144 |
| Chromosome: | chromosome 6 |
| Location: | 8329955 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g306250 | Hypothetical protein; (1 of 9) 2.7.10.1 - Receptor protein-tyrosine kinase / Receptor protein tyrosine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATGCCTGACGAGGGGCTCGGCCGGGAT |
| Internal bar code: | ACAGTTGATTTTTGTTATTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 99.6437 |
| LEAP-Seq n confirming: | 4474 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATTGTTGCAGACGGATGGA |
| Suggested primer 2: | CCTGCAGCTCCTGTACTTCC |