| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183174 |
| Chromosome: | chromosome 7 |
| Location: | 2872161 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332300 | GWD2,1 | Alpha-glucan water dikinase 2; (1 of 3) 2.7.9.4 - Alpha-glucan, water dikinase / Starch-related R1 protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGAACACCTGCCAACCACACCAGCCTG |
| Internal bar code: | GCGTCGTCTGTGGGGTTCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 263 |
| LEAP-Seq percent confirming: | 98.0548 |
| LEAP-Seq n confirming: | 1361 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATGGGCCATCGAGTCTTA |
| Suggested primer 2: | TAGTGCTTGAAGGGCTCGAT |