| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183189 |
| Chromosome: | chromosome 6 |
| Location: | 7069313 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297016 | (1 of 1) K10843 - DNA excision repair protein ERCC-3 (ERCC3, XPB) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTTTTGTGCAGGGCGTGAGTTCAGGAAT |
| Internal bar code: | AAGGTCCATCACTAACCAAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 7 |
| LEAP-Seq percent confirming: | 99.6241 |
| LEAP-Seq n confirming: | 2120 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCACCACCTCGATGTTCC |
| Suggested primer 2: | ACTTCACCGTTCACACCCTC |