| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183203 |
| Chromosome: | chromosome 9 |
| Location: | 912796 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401150 | NUOFAF2 | (1 of 1) K18160 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 2 (NDUFAF2); NADH:ubiquinone oxidoreductase 1 alpha subcomplex assembly factor 2 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAACCAAAGCGACGTGGGGCGGCGATGG |
| Internal bar code: | ACCTAGCGACGAAGTAGACGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 396 |
| LEAP-Seq percent confirming: | 97.8979 |
| LEAP-Seq n confirming: | 1304 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTAATGCACCCGTGACCT |
| Suggested primer 2: | CAAGTGTCCGGAATTTCGTT |