Insertion junction: LMJ.RY0402.183207_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.183207
Chromosome:chromosome 6
Location:1498510
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g259650 MPA7 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR10161 - TARTRATE-RESISTANT ACID PHOSPHATASE TYPE 5 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGGCACGACTGACAACTTTGCTCCGCC
Internal bar code:GTATCCATCACGGGGAACAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:100
LEAP-Seq percent confirming:99.6219
LEAP-Seq n confirming:1054
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGCGGTATCCCACAGAAC
Suggested primer 2:CCTCACTGGAGGCTTCACTC