| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183209 |
| Chromosome: | chromosome 1 |
| Location: | 2610959 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015150 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGTCGCTGACTGCCTCAGCGTCCTCC |
| Internal bar code: | GTCCTATTAGGATGGGGCGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 95.1786 |
| LEAP-Seq n confirming: | 533 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTACTGTCTCCACGCAGC |
| Suggested primer 2: | GCTAATCTGTGTCCCGTGGT |