| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183255 |
| Chromosome: | chromosome 4 |
| Location: | 3765478 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g229518 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTAAAAAGCCGCACCTCCGTCTATGTTGG |
| Internal bar code: | CCTGGTTGCTTTTATGGCAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 86.2219 |
| LEAP-Seq n confirming: | 2572 |
| LEAP-Seq n nonconfirming: | 411 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACACCG |
| Suggested primer 2: | GCGTGTGTGTGTGTGTGTGT |