| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183261 |
| Chromosome: | chromosome 12 |
| Location: | 8769307 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g545600 | (1 of 4) IPR001841//IPR018957 - Zinc finger, RING-type // Zinc finger, C3HC4 RING-type | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCATCTCTCCGACACTGGTCGCTTTTT |
| Internal bar code: | CTTCCGGCTATCCGCCCGGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1088 |
| LEAP-Seq percent confirming: | 99.7959 |
| LEAP-Seq n confirming: | 1956 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAACCACTACGAAACCA |
| Suggested primer 2: | TTGTTCCTCGTGCTATGCTG |