| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183265 |
| Chromosome: | chromosome 8 |
| Location: | 4942678 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g385400 | (1 of 3) 3.5.1.1 - Asparaginase / L-asparagine amidohydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCGCATCGCCCCTGCCCCTTGCAGTGA |
| Internal bar code: | ATGGGCTCCGCGGGAGTCTATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 99.8122 |
| LEAP-Seq n confirming: | 2658 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCGCATGACCTATCAGCA |
| Suggested primer 2: | GTCTCCTTCCAGTCGCAAAG |