| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183272 |
| Chromosome: | chromosome 7 |
| Location: | 2255026 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327500 | (1 of 3) PF10358 - N-terminal C2 in EEIG1 and EHBP1 proteins (NT-C2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGACTAGTCAGCGCATCTGCCACGCCCC |
| Internal bar code: | CACCGCTGTGGTTTAAAGGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 700 |
| LEAP-Seq percent confirming: | 99.6449 |
| LEAP-Seq n confirming: | 1964 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGAGACATATCACCAGCA |
| Suggested primer 2: | CATGCAGGTGTATGGCTGTC |