| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.183313 |
| Chromosome: | chromosome 9 |
| Location: | 6855627 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g409951 | RuvX homolog; (1 of 1) K07447 - putative holliday junction resolvase (ruvX) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTAATTCTTTTAGGCAGCGTCCTGCTTCC |
| Internal bar code: | ATGAAACGATCTTGAACTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 98.5258 |
| LEAP-Seq n confirming: | 401 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGTCAGTCAGCCTTAGTC |
| Suggested primer 2: | CTGCAGATGAGAAGAGCACG |