| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183340 |
| Chromosome: | chromosome 14 |
| Location: | 306131 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609900 | (1 of 2) PTHR23130//PTHR23130:SF61 - FAMILY NOT NAMED // DEFENSE PROTEIN L(2)34FC | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCGTTTCCGACTTCTAGCGGGTCAGAGC |
| Internal bar code: | GTTCACGCCTTCACCCTGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 99.5876 |
| LEAP-Seq n confirming: | 1449 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGCAGACTCACCCTTGGC |
| Suggested primer 2: | AAAAGTCCGTTTGCATGAGG |