| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183345 |
| Chromosome: | chromosome 10 |
| Location: | 1800066 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g430950 | RAD54B,SRH18 | (1 of 1) K10875 - DNA repair and recombination protein RAD54 and RAD54-like protein (RAD54L, RAD54); SNF2-related DNA/RNA helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCGGCGGCGGCGTAGGCGGCTGGCGC |
| Internal bar code: | CGTATGCGCTTGCAGAGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGTATTTCGCTGTCGCA |
| Suggested primer 2: | CGTACTGGATAGGGAAGCCA |