Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.183385 |
Chromosome: | chromosome 5 |
Location: | 2113746 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre05.g234640 | (1 of 1) IPR000048//IPR011989//IPR016024 - IQ motif, EF-hand binding site // Armadillo-like helical // Armadillo-type fold | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGTGTAGCGAAGTAGGGCTGGGGGGGGG |
Internal bar code: | ACGAAGCCACGGGGACCGACTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 377 |
LEAP-Seq percent confirming: | 95.0549 |
LEAP-Seq n confirming: | 173 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCACAGCAGACTTACCCAC |
Suggested primer 2: | TATGTGGAGTTCCTGGAGGC |