| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.183386 |
| Chromosome: | chromosome 12 |
| Location: | 3062400 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500450 | TPD1 | (1 of 2) PTHR12121:SF37 - 2',5'-PHOSPHODIESTERASE 12; 2'%252C5'-phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACGCTAGGGCACCAGCCCGCGAGAA |
| Internal bar code: | TAGCTTATCAGCTCCAGAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 238 |
| LEAP-Seq percent confirming: | 97.9339 |
| LEAP-Seq n confirming: | 237 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCAACCACGAGTGTAACC |
| Suggested primer 2: | TAGGGGTGGAAGAACAGGTG |