| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.183389 |
| Chromosome: | chromosome 8 |
| Location: | 5014659 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g386050 | (1 of 1) PTHR12917//PTHR12917:SF11 - ASPARTYL PROTEASE DDI-RELATED // RHOMBOID DOMAIN-CONTAINING PROTEIN 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTTCAAGGCGTGACAAAATGTACTTCT |
| Internal bar code: | GGAGGATCTAAGTCACTGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 977 |
| LEAP-Seq percent confirming: | 93.8723 |
| LEAP-Seq n confirming: | 3309 |
| LEAP-Seq n nonconfirming: | 216 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGTTGCTGCCAGATTCC |
| Suggested primer 2: | GTGAATGGGCAACATGTCAG |