Insertion junction: LMJ.RY0402.183400_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.183400
Chromosome:chromosome 17
Location:4512211
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre17.g732533 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGTAGCAGCATTGTATGCAGTCCGGGGG
Internal bar code:GGTGATCATCTGTCGCGTGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:850
LEAP-Seq percent confirming:99.3907
LEAP-Seq n confirming:2773
LEAP-Seq n nonconfirming:17
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:ACACAAACACAACCGCACAT
Suggested primer 2:CGCCTACTAGCACATCGTCA