| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183423 |
| Chromosome: | chromosome 12 |
| Location: | 8357978 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549250 | (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGGCTTCTGGTGCAACCCATTTCAAGCT |
| Internal bar code: | GGGGCGAGAATGTCGAAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 93.6042 |
| LEAP-Seq n confirming: | 2488 |
| LEAP-Seq n nonconfirming: | 170 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCCTGTCCATCTTCCTGTA |
| Suggested primer 2: | GCGGAGCTGCTGTATGTGTA |