| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183433 |
| Chromosome: | chromosome 7 |
| Location: | 1719515 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325711 | (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCAAGAGAAGTGGCATGCGGTGGCTTA |
| Internal bar code: | TTGTGCTTGCACACCGTGTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 69.0832 |
| LEAP-Seq n confirming: | 324 |
| LEAP-Seq n nonconfirming: | 145 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGCTACAGTCTCCCAGCA |
| Suggested primer 2: | CCTGCCAATGCTTACCGTAT |