Insertion junction: LMJ.RY0402.183470_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.183470
Chromosome:chromosome 10
Location:468498
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g420950 (1 of 45) IPR029052 - Metallo-dependent phosphatase-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACAATCATCCCGGTCCGCTTCCCGCCCT
Internal bar code:CTGACATTCATTAAAAAAATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:29
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:99
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTACGTCGCTGGACTAC
Suggested primer 2:TAGCCAGTGTACCCCTGACC