Insertion junction: LMJ.RY0402.183480_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.183480
Chromosome:chromosome 11
Location:3184973
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g479383 (1 of 1) IPR000104//IPR013122 - Antifreeze protein, type I // Polycystin cation channel, PKD1/PKD2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCGAACAGGCTGCCGTACGACGCGGCCA
Internal bar code:CACCGACAGACCCGGCTAGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:551
LEAP-Seq percent confirming:90.4701
LEAP-Seq n confirming:712
LEAP-Seq n nonconfirming:75
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGGATACCATGTGGCTG
Suggested primer 2:TCAACAGCTCGACTGAAACG