Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.183482 |
Chromosome: | chromosome_3 |
Location: | 735971 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g146127 | FAP96 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGAGTAGTGGCCCCAGCCATATTCAAAAAG |
Internal bar code: | ACCAGCATCTTGGCGCCCGTTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 907 |
LEAP-Seq percent confirming: | 97.5911 |
LEAP-Seq n confirming: | 1661 |
LEAP-Seq n nonconfirming: | 41 |
LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACTCTACACACCGTCCCGTC |
Suggested primer 2: | TGCGCATGAACCTCAAGTAG |