| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183482 |
| Chromosome: | chromosome_5 |
| Location: | 1960927 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g241637 | FAP32 | Intraflagellar transport protein 46 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAGCAGGTCTTGCGAAAACGCAACTCCGGG |
| Internal bar code: | AATTGCGCGCGCTCATATCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 99.7748 |
| LEAP-Seq n confirming: | 3987 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGATGAGGGGTGTTACG |
| Suggested primer 2: | TGGCTACGCCTTCACTTCTT |