| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183505 |
| Chromosome: | chromosome 13 |
| Location: | 3637406 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588626 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCAACCCTTTCAAGCTTGACTCACATT |
| Internal bar code: | GGGGGTTACGGTAAGCGAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 96.9925 |
| LEAP-Seq n confirming: | 3225 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCTGCATTCAAAAGCTA |
| Suggested primer 2: | TCTAGGAAACGGCCAAAATG |