Insertion junction: LMJ.RY0402.183511_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.183511
Chromosome:chromosome 13
Location:3161576
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g585250 (1 of 1) PF08005 - PHR domain (PHR) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACGAGCTCACCACGAAGGCGCACCGTGC
Internal bar code:TTGCTCTTCGGGTATAGTGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:30
LEAP-Seq percent confirming:51.8519
LEAP-Seq n confirming:126
LEAP-Seq n nonconfirming:117
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTGCAACATAATGAGCTCGC
Suggested primer 2:TGATAATTCCGACTCCCCTG