Insertion junction: LMJ.RY0402.183519_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.183519
Chromosome:chromosome 9
Location:2070889
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g394050 CPLD19 Zinc finger family protein; (1 of 1) PTHR22883:SF91 - PROTEIN S-ACYLTRANSFERASE 12-RELATED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTGTCTCGCTTCTGACCTGTACCGTCAA
Internal bar code:GACGACCGATCGGGGAACGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:576
LEAP-Seq percent confirming:80.0106
LEAP-Seq n confirming:1509
LEAP-Seq n nonconfirming:377
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTCCCCTTCCTTCCTTC
Suggested primer 2:CGATTTAGTCCAGCGACCAT