Insertion junction: LMJ.RY0402.183530_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.183530
Chromosome:chromosome 7
Location:1718860
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g325711 (1 of 2) K19363 - lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGTGATTGTGGGTAGATGTGGGACAAGA
Internal bar code:AAGGTGGGTCGAGCCAGGGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:35
LEAP-Seq percent confirming:86.1111
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TACCTGTCGCACACACCATT
Suggested primer 2:TGCCATCTATTGCTGTCTGC