| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183542 |
| Chromosome: | chromosome 13 |
| Location: | 3965248 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g590900 | APC11 | Anaphase promoting complex subunit 11 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTTATGTGGCGTGGCGGTGTGTATCATG |
| Internal bar code: | GGGTTTCTTGGGTGGAGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1003 |
| LEAP-Seq percent confirming: | 99.7914 |
| LEAP-Seq n confirming: | 11957 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCTGCTGGTGAGTTCCT |
| Suggested primer 2: | TTGGAGTTGGGGCTATCAAG |