| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.183551 |
| Chromosome: | chromosome 17 |
| Location: | 4928318 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g734548 | PPD2 | Pyruvate phosphate dikinase; (1 of 1) 2.7.11.32//2.7.9.1 - [Pyruvate, phosphate dikinase] kinase / Pyruvate, phosphate dikinase regulatory protein // Pyruvate, phosphate dikinase / Pyruvate,phosphate dikinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAACACTATGTCAATGTGGATACTTCCCC |
| Internal bar code: | AGCTTACGGGACGATTTTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1190 |
| LEAP-Seq percent confirming: | 99.5146 |
| LEAP-Seq n confirming: | 1230 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTGCTTGCACATTGAGA |
| Suggested primer 2: | GCTGGTGTATGTGTGTTGGC |