| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.183553 |
| Chromosome: | chromosome 13 |
| Location: | 907010 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567800 | PPP41,PP2A2 | (1 of 1) K11584 - serine/threonine-protein phosphatase 2A regulatory subunit B' (PPP2R5); Protein phosphatase 2A regulatory B subunit | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACCTACCACGTGCTTTACCTGTTGCTC |
| Internal bar code: | ATGAGTATGCCGCAGGACTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 98.452 |
| LEAP-Seq n confirming: | 636 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCAGAGCTGATCGTTT |
| Suggested primer 2: | CGCAGGAGGAACTCGTAGAC |